Supplementary Data To Galkin et al., "ADF Stabilizes 
an Existing State of F-actin and Can Change the Tilt 
of F-actin Subunits". J. Cell Biol., 2001, Vol. 153, pp. 75-86

Three actin subunits and three of each first and second 
bound h-ADF are included in the UNIX-compressed PDB 
file "three_subunits.pdb".

Note that actin and ADF subunits were fitted to the EM 
data individually (helical symmetry was applied). 
Therefore, no effort was made to alleviate steric 
clashes. In particular, the N-terminus of the first 
ADF overlaps with actin. This is consistent with 
[Hatanaka et al., Cell 85 pp. 1047, 1996], where such 
a clash was first reported in their model. Molecular 
dynamics simulations of cofilin revealed a highly 
flexible N-terminus that required remodeling to 
alleviate the steric clash with G-actin [Wriggers 
et al., J. Molecular Biology 282 pp. 921, 1998].

Last update: May 16, 2001.

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In case of problems or questions please contact
Willy Wriggers (wriggers "at" biomachina.org)